产品名称
Pig porcine N-Cadherin/CDH2 ELISA Kit
指标别名
Cadherin 2; CD325; CDH2; CDHN; CDw325; N cadherin; NCAD; N-cadherin; Neural cadherin
免疫原
Expression system for standard: NS0; Immunogen sequence: D160-A724
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Pig porcine N-Cadherin/CDH2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0669-PO)
背景资料
Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
试剂盒内容
Catalog number | Description | Quantity |
EK0669-PO-CAP | Anti-Pig CDH2 Pre-coated 96-well strip microplate | 1 |
EK0669-PO-ST | Pig CDH2 Standard | 50ng/tube×2 |
EK0669-PO-DA | Pig CDH2 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (ng/ml) | 1.23 | 3.46 | 6.92 | 1.38 | 3.57 | 6.85 |
Standard deviation | 0.059 | 0.180 | 0.388 | 0.073 | 0.203 | 0.425 |
CV (%) | 4.8 | 5.2 | 5.6 | 5.3 | 5.7 | 6.2 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.025 | 0.075 | 0.119 | 0.210 | 0.369 | 0.749 | 1.355 | 2.007 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)