产品名称
Mouse N-Cadherin/CDH2 ELISA Kit
指标别名
Cadherin 2; CD325; CDH2; CDHN; CDw325; N cadherin; NCAD; N-cadherin; Neural cadherin
免疫原
Expression system for standard: NS0; Immunogen sequence: D160-A724
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse N-Cadherin/CDH2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1155)
背景资料
Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1155-CAP | 96-well plate precoated with anti- mouse Cadherin-2 antibody | 1 |
EK1155-ST | Lyophilized recombinant mouse Cadherin-2 standard | 50ng/tube×2 |
EK1155-DA | Biotinylated anti- mouse Cadherin-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1737 | 5669 | 22081 | 1767 | 5460 | 20442 |
Standard deviation | 74.69 | 340.14 | 1236.53 | 102.48 | 425.88 | 1512.7 |
CV(%) | 4.3% | 6% | 5.6% | 5.8% | 7.8% | 7.4% |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.081 | 0.181 | 0.249 | 0.456 | 0.675 | 1.284 | 1.864 | 2.330 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)