产品名称
Human TARC/CCL17 ELISA Kit
指标别名
A 152E5.3; ABCD 2; C C motif chemokine 17; CC chemokine TARC; CCL17; SCYA17; Small inducible cytokine A17; TARC
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
chemokine (C-C motif) ligand 17
文献引用格式
Human TARC/CCL17 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0684)
背景资料
Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0684-CAP | 96-well plate precoated with anti- human CCL17 antibody | 1 |
EK0684-ST | Lyophilized recombinant human CCL17 standard | 10ng/tubex2 |
EK0684-DA | Biotinylated anti- human CCL17 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 62 | 251 | 939 | 56 | 234 | 931 |
Standard deviation | 2.6 | 10.79 | 53.52 | 2.68 | 12.16 | 62.37 |
CV(%) | 4.2 | 4.3 | 5.7 | 4.8 | 5.2 | 6.7 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.088 | 0.091 | 0.152 | 0.257 | 0.610 | 0.939 | 1.543 | 1.947 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)