产品名称
Mouse TARC/CCL17 Quick ELISA Kit
指标别名
A 152E5.3; ABCD 2; C C motif chemokine 17; CC chemokine TARC; CCL17; SCYA17; Small inducible cytokine A17; TARC
免疫原
Expression system for standard: E.coli; Immunogen sequence: A24-P93
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 17
文献引用格式
Mouse TARC/CCL17 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0685)
背景资料
Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.
试剂盒内容
Catalog number | Description | Quantity |
FEK0685-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK0685-ST | Mouse TARC standard | 10ng/tube×2 |
FEK0685-A | Mouse TARC Antibody A | 3ml |
FEK0685-B | Mouse TARC Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 64 | 214 | 1001 | 60 | 231 | 1081 |
Standard deviation | 3.26 | 11.77 | 62.06 | 3.96 | 17.09 | 83.23 |
CV(%) | 5.1% | 5.5% | 6.2% | 6.6% | 7.4% | 7.7% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
O.D. | 0.051 | 0.135 | 0.269 | 0.447 | 0.761 | 1.298 | 1.998 | 2.321 |
特异性
Natural and recombinant mouse Ccl17
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)