产品名称
Human MIP-4/CCL18 ELISA Kit
指标别名
AMAC 1; AMAC1; C C motif chemokine 18; CC chemokine PARC; CCL18; CKb7; DC CK1; DCCK1; Dendritic cell chemokine 1; MIP 4; MIP4; PARC; SCYA18; Small inducible cytokine A18
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
基因名全称
chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
文献引用格式
Human MIP-4/CCL18 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0686)
背景资料
Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0686-CAP | 96-well plate precoated with anti- human CCL18 antibody | 1 |
EK0686-ST | Lyophilized recombinant human CCL18 standard | 10ng/tubex2 |
EK0686-DA | Biotinylated anti- human CCL18 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 49 | 269 | 18 | 52 | 279 |
Standard deviation | 0.99 | 3.52 | 20.17 | 1.29 | 4.62 | 22.59 |
CV(%) | 5.5 | 7.2 | 7.5 | 7.2 | 8.9 | 8.1 |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.006 | 0.045 | 0.075 | 0.186 | 0.377 | 0.754 | 1.505 | 2.145 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum