产品名称
Human CCL18/PARC EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
AMAC 1; AMAC1; C C motif chemokine 18; CC chemokine PARC; CCL18; CKb7; DC CK1; DCCK1; Dendritic cell chemokine 1; MIP 4; MIP4; PARC; SCYA18; Small inducible cytokine A18
免疫原
Expression system for standard: E.coli; Immunogen sequence: A21-A89
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
基因名全称
C-C motif chemokine ligand 18
文献引用格式
Human CCL18/PARC EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0686)
背景资料
Chemokine(C-C motif) ligand 18(CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC(pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ0686-CA |
Rabbit anti- human CCL18 polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ0686-DA |
Biotinylated goat anti- human CCL18 polyclonal antibody (Detection Antibody) |
500 µl, 2.5 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ0686-ST |
Lyophilized recombinant human CCL18 standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.006 | 0.045 | 0.075 | 0.186 | 0.377 | 0.754 | 1.505 | 2.145 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
蛋白名全称
C-C motif chemokine 18
样本类型
Cell Culture Supernatants and Serum
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