Human CD14 ELISA Kit

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EK0694

96T ￥2480
货期：现货

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Human CD14 ELISA Kit standard curve
all(2)
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产品简介>

产品名称

Human CD14 ELISA Kit

规格/价格

96T/2480

指标别名

CD14; CD14 molecule

免疫原

CHO, T20-C352

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

CD14

基因名全称

CD14 molecule

Uniprot ID

P08571

文献引用格式

Human CD14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0694)

背景资料

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0694-CAP	96-well plate precoated with anti- human CD14 antibody	1
EK0694-ST	Lyophilized recombinant human CD14 standard	10ng/tube×2
EK0694-DA	Biotinylated anti- human CD14 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	310	1057	4116	312	1164	4732
Standard deviation	13.95	49.7	209.92	18.1	75.66	350.2
CV(%)	4.5	4.7	5.1	5.8	6.5	7.4

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.079	0.137	0.191	0.328	0.567	0.973	1.612	2.313

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human CD14 ELISA Kit

EK0694

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

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Human CD14 ELISA Kit

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