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Mouse CD14 ELISA Kit

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EK0695

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse CD14 ELISA Kit
规格/价格
96T/2480
指标别名
CD14; CD14 molecule
免疫原
Expression system for standard: NS0; Immunogen sequence: A18-P345
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
0.78ng/ml-50ng/ml
基因名称
CD14
基因名全称
Monocyte differentiation antigen CD14
Uniprot ID
P10810  
文献引用格式
Mouse CD14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0695)
背景资料
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
试剂盒内容
Catalog numberDescriptionQuantity
EK0695-CAP96-well plate precoated with anti- mouse CD14 antibody1
EK0695-STLyophilized recombinant mouse CD14 standard50ng/tubex2
EK0695-DABiotinylated anti- mouse CD14 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)18094760243401781430524426
Standard deviation103.11261.81217119.32236.771392.28
CV(%)5.7%5.5%5%6.7%5.5%5.7%
典型数据示例
Concentration(ng/ml)00.781.563.126.2512.52550
O.D.0.0690.1350.1800.2740.4750.8581.2832.118
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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