产品名称
Mouse CD14 ELISA Kit
免疫原
Expression system for standard: NS0; Immunogen sequence: A18-P345
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Monocyte differentiation antigen CD14
文献引用格式
Mouse CD14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0695)
背景资料
CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0695-CAP | 96-well plate precoated with anti- mouse CD14 antibody | 1 |
EK0695-ST | Lyophilized recombinant mouse CD14 standard | 50ng/tubex2 |
EK0695-DA | Biotinylated anti- mouse CD14 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1809 | 4760 | 24340 | 1781 | 4305 | 24426 |
Standard deviation | 103.11 | 261.8 | 1217 | 119.32 | 236.77 | 1392.28 |
CV(%) | 5.7% | 5.5% | 5% | 6.7% | 5.5% | 5.7% |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.069 | 0.135 | 0.180 | 0.274 | 0.475 | 0.858 | 1.283 | 2.118 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)