指标别名
IL 7; IL7; IL-7; interleukin 7
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
7.8 pg/ml - 500 pg/ml
文献引用格式
Human IL7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0779)
背景资料
IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0779-CAP | 96-well plate precoated with anti- human IL-7 antibody | 1 |
EK0779-ST | Lyophilized recombinant human IL-7 standard | 10ng/tube×2 |
EK0779-DA | Biotinylated anti- human IL-7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 17 | 81 | 209 | 15 | 78 | 192 |
Standard deviation | 0.98 | 5.1 | 9.82 | 1 | 5.38 | 10.56 |
CV(%) | 5.8 | 6.3 | 4.7 | 6.7 | 6.9 | 5.5 |
典型数据示例
Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.022 | 0.066 | 0.115 | 0.217 | 0.461 | 0.966 | 1.605 | 2.331 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA)