Catalog number | Description | Quantity |
---|---|---|
EK0780-CAP | 96-well plate precoated with anti- mouse IL-7 antibody | 1 |
EK0780-ST | Lyophilized recombinant mouse IL-7 standard | 10ng/tube×2 |
EK0780-DA | Biotinylated anti- mouse IL-7 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 51 | 173 | 551 | 56 | 201 | 599 |
Standard deviation | 2.33 | 4.67 | 39.07 | 3.32 | 12.96 | 38.04 |
CV(%) | 4.6% | 2.7% | 7.1% | 5.9% | 6.5% | 6.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.080 | 0.146 | 0.234 | 0.310 | 0.486 | 0.636 | 0.993 | 1.747 |
Mouse IL-7 ELISA Kit standard curve
Mouse IL-7 ELISA Kit standard curve