产品名称
Human MER/MERTK ELISA Kit
指标别名
MER proto-oncogene; tyrosine kinase; MER; RP38; Tyro12; c-Eyk; c-mer; MERTK
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
c-mer proto-oncogene tyrosine kinase
文献引用格式
Human MER/MERTK ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0810)
背景资料
Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type(immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium(RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa(RP)
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0810-CAP | 96-well plate precoated with anti- human Mer antibody | 1 |
| EK0810-ST | Lyophilized recombinant human Mer standard | 10ng/tube×2 |
| EK0810-DA | Biotinylated anti- human Mer antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 236 | 1665 | 4937 | 217 | 1701 | 4920 |
| Standard deviation | 14.63 | 79.92 | 197.48 | 13.67 | 105.46 | 221.4 |
| CV(%) | 6.2 | 4.8 | 4 | 6.3 | 6.2 | 4.5 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.039 | 0.115 | 0.181 | 0.335 | 0.584 | 1.093 | 1.734 | 2.219 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates and Serum