产品名称
Mouse MER/MERTK ELISA Kit
指标别名
Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; Mertk; Mer
免疫原
Expression system for standard: NS0; Immunogen sequence: E23-F498
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tyrosine-protein kinase Mer
文献引用格式
Mouse MER/MERTK ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0811)
背景资料
Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0811-CAP | 96-well plate precoated with anti- mouse MER antibody | 1 |
EK0811-ST | Lyophilized recombinant mouse MER standard | 10ng/tube×2 |
EK0811-DA | Biotinylated anti- mouse MER antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 109 | 571 | 2250 | 114 | 562 | 2147 |
Standard deviation | 6.86 | 30.83 | 132.75 | 8.55 | 39.34 | 145.99 |
CV(%) | 6.3% | 5.4% | 5.9% | 7.5% | 7% | 6.8% |
典型数据示例
Concentration(pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.019 | 0.063 | 0.114 | 0.192 | 0.366 | 0.663 | 1.167 | 2.133 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates and Serum