Human MIF ELISA Kit

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EK0813

96T ￥2480
货期：现货

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Human MIF ELISA Kit standard curve
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产品简介>

产品名称

Human MIF ELISA Kit

规格/价格

96T/2480

指标别名

GIF; GLIF; L dopachrome isomerase; L dopachrome tautomerase; MIF; MMIF; Phenylpyruvate tautomerase

免疫原

E.coli, P2-A115

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

MIF

基因名全称

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Uniprot ID

P14174

文献引用格式

Human MIF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0813)

背景资料

Macrophage migration inhibitory factor(MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0813-CAP	96-well plate precoated with anti- human MIF antibody	1
EK0813-ST	Lyophilized recombinant human MIF standard	10ng/tube×2
EK0813-DA	Biotinylated anti- human MIF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	280	1655	4559	305	1694	4885
Standard deviation	12.04	66.2	205.15	16.47	77.92	307.75
CV(%)	4.3	4	4.5	5.4	4.6	6.3

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.046	0.111	0.215	0.357	0.635	1.063	1.582	1.999

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human MIF ELISA Kit

EK0813

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

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Human MIF ELISA Kit

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