产品名称
Monkey primate MIF ELISA Kit
指标别名
GIF; GLIF; L dopachrome isomerase; L dopachrome tautomerase; MIF; MMIF; Phenylpyruvate tautomerase
免疫原
Expression system for standard: E.coli; Immunogen sequence: P2-A115
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
文献引用格式
Monkey primate MIF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0813-PR)
背景资料
Macrophage migration inhibitory factor(MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to monkey primate chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.
试剂盒内容
Catalog number | Description | Quantity |
EK0813-PR-CAP | Anti-Monkey MIF Pre-coated 96-well strip microplate | 1 |
EK0813-PR-ST | Monkey MIF Standard | 10ng/tube×2 |
EK0813-PR-DA | Monkey MIF Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1442 | 3826 | 6427 | 1625 | 4177 | 6782 |
Standard deviation | 90.85 | 206.6 | 315 | 121.9 | 254.8 | 393.4 |
CV (%) | 6.1 | 5.4 | 4.9 | 7.5 | 6.1 | 5.8 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
O.D. | 0.046 | 0.111 | 0.215 | 0.357 | 0.635 | 1.063 | 1.582 | 1.999 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and monkey primate milk