产品名称
Human IL23 ELISA Kit
指标别名
IL 23; IL 23 A; IL 23 subunit alpha; IL 23A; IL 23p19; IL23A; IL-23A; IL23P19; Interleukin 23 subunit alpha; Interleukin 23 subunit p19; P19; SGRF
免疫原
Expression system for standard: NS0; Immunogen sequence: (R20-P189)+(I23-S328)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
基因名全称
interleukin 23 subunit alpha
文献引用格式
Human IL23 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0865)
背景资料
This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
试剂盒内容
Catalog number | Description | Quantity |
EK0865-CAP | 96-well plate precoated with anti- Human IL-23 ELISA Kit A antibody | 1 |
EK0865-ST | Lyophilized recombinant Human IL-23 ELISA Kit standard | 10ng/tube×2 |
EK0865 -DA | Biotinylated anti- Human IL-23 ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 280 | 519 | 4052 | 195 | 572 | 2736 |
Standard deviation | 17.22 | 14.12 | 142.63 | 8.09 | 15.44 | 98.5 |
CV(%) | 6.2% | 2.7% | 3.5% | 4.2% | 2.7% | 3.6% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
O.D. | 0.053 | 0.176 | 0.292 | 0.412 | 0.645 | 0.978 | 1.241 | 2.043 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA).