产品名称
Mouse IL23/IL23A ELISA Kit
指标别名
IL 23; IL 23 A; IL 23 subunit alpha; IL 23A; IL 23p19; IL23A; IL-23A; IL23P19; Interleukin 23 subunit alpha; Interleukin 23 subunit p19; P19; SGRF
免疫原
Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (L20-A196)
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Interleukin-23 subunit alpha
文献引用格式
Mouse IL23/IL23A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0866)
背景资料
Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0866-CAP | 96-well plate precoated with anti- mouse IL-23 antibody | 1 |
| EK0866-ST | Lyophilized recombinant mouse IL-23 standard | 10ng/tube×2 |
| EK0866-DA | Biotinylated anti- mouse IL-23 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 93 | 278 | 780 | 85 | 279 | 762 |
| Standard deviation | 4.09 | 13.9 | 34.32 | 5.27 | 19.25 | 38.86 |
| CV(%) | 4.4% | 5% | 4.4% | 6.2% | 6.9% | 5.1% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.043 | 0.115 | 0.175 | 0.268 | 0.446 | 0.823 | 1.418 | 2.273 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)