Catalog number | Description | Quantity |
---|---|---|
EK0906-CAP | 96-well plate precoated with anti- human TLR2 antibody | 1 |
EK0906-ST | Lyophilized recombinant human TLR2 standard | 10ng/tube×2 |
EK0906-DA | Biotinylated anti- human TLR2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 369 | 2018 | 5303 | 392 | 1927 | 5643 |
Standard deviation | 18.81 | 133.18 | 212.12 | 22.34 | 150.3 | 242.64 |
CV(%) | 5.1 | 6.6 | 4 | 5.7 | 7.8 | 4.3 |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.088 | 0.108 | 0.180 | 0.292 | 0.431 | 0.988 | 1.331 | 2.147 |
Human TLR2 ELISA Kit standard curve
Human TLR2 ELISA Kit standard curve