Catalog number | Description | Quantity |
---|---|---|
EK1130-CAP | 96-well plate precoated with anti- mouse TLR2 antibody | 1 |
EK1130-ST | Lyophilized recombinant mouse TLR2 standard | 10ng/tube×2 |
EK1130-DA | Biotinylated anti- mouse TLR2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 304 | 1448 | 4661 | 301 | 1423 | 4925 |
Standard deviation | 21.58 | 65.16 | 195.76 | 26.48 | 91.07 | 280.72 |
CV(%) | 7.1% | 4.5% | 4.2% | 8.8% | 6.4% | 5.7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.016 | 0.078 | 0.128 | 0.200 | 0.412 | 0.853 | 1.434 | 2.097 |
Mouse TLR2 ELISA Kit standard curve
Mouse TLR2 ELISA Kit standard curve