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Bovine Endothelin ELISA Kit

说明书

EK0945-BV

  • 96T ¥2480
  • 货期: 现货

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产品名称
Bovine Endothelin ELISA Kit
规格/价格
96T/2480
指标别名
EDN1; EDN2; EDN3; endothelin 1/2/3; ET 1/2/3 Big endothelin 1; ET1; ET2; ET3; HDLCQ7; PPET1; PPET2; PPET3; Preproendothelin 1/2/3
免疫原
A peptide reflecting the target protein in bovine. Sequence: C53-W73
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.9pg/ml-250pg/ml
基因名称
EDN1/EDN2/EDN3
基因名全称
endothelin 1; endothelin 2; endothelin 3
Uniprot ID
文献引用格式
Bovine Endothelin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0945-BV)
背景资料
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Bovine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0945-BV-CAPAnti-Bovine EDN1 Pre-coated 96-well strip microplate1
EK0945-BV-STBovine EDN1 Standard1ng/tube×2
EK0945-BV-DABovine EDN1 Biotinylated antibody (100x)100ul
AR1103Avidin-Biotin-Peroxidase Complex (100x)100ul
AR1106-1Sample Diluent30ml
AR1106-2Antibody Diluent12ml
AR1106-3Avidin-Biotin-Peroxidase Diluent12ml
AR1104Color Developing Reagent (TMB)10ml
AR1105Stop solution10ml
AR1106-5Wash Buffer (25x)20ml
PLA-SEAAdhesive plate sealers4
批内差/批间差
Sample123123
n161616242424
Mean (pg/ml)26.185.414623.284.3143
Standard deviation1.413.768.031.796.078.72
CV (%)4.4%4.4%5.5%7.7%7.2%6.1%
典型数据示例
Concentration(pg/ml)03.97.815.631.262.5125250
O.D.0.0170.0450.0880.1570.3570.7721.6462.039
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
bovine
蛋白名全称
Endothelin-1; Endothelin-2; Endothelin-3
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

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