Chinese Hamster Endothelin ELISA Kit

96T/2480

EDN1; EDN2; EDN3; endothelin 1/2/3; ET 1/2/3 Big endothelin 1; ET1; ET2; ET3; HDLCQ7; PPET1; PPET2; PPET3; Preproendothelin 1/2/3

A peptide reflecting the target protein in chinese hamster. Sequence: C53-W73

There is no detectable cross-reactivity with other relevant proteins.

<0.5pg/ml

3.9pg/ml-250pg/ml

EDN1/EDN2/EDN3

endothelin 1; endothelin 2; endothelin 3

G3H0G9

Chinese Hamster Endothelin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0945-HA)

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Chinese hamster endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

20-25分钟

Catalog number	Description	Quantity
EK0945-HA-CAP	Anti-Hamster EDN1 Pre-coated 96-well strip microplate	1
EK0945-HA-ST	Hamster EDN1 Standard	1ng/tube×2
EK0945-HA-DA	Hamster EDN1 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	26.1	85.4	146	23.2	84.3	143
Standard deviation	1.41	3.76	8.03	1.79	6.07	8.72
CV (%)	4.4	4.4	5.5	7.7	7.2	6.1

Concentration(pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.019	0.047	0.085	0.178	0.410	0.879	1.813	2.570

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

hamster

Endothelin-1; Endothelin-2; Endothelin-3

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)