指标别名
C reactive protein; CRP; PTX1
免疫原
Expression system for standard: NS0; Immunogen sequence: H20-S230
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-reactive protein, pentraxin-related
文献引用格式
Rat CRP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0978)
背景资料
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
试剂盒内容
Catalog number | Description | Quantity |
EK0978-CAP | 96-well plate precoated with anti- rat CRP antibody | 1 |
EK0978-ST | Lyophilized recombinant rat CRP standard | 10ng/tube×2 |
EK0978-DA | Biotinylated anti- rat CRP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 225 | 1505 | 4812 | 235 | 1505 | 4457 |
Standard deviation | 11.02 | 81.27 | 307.96 | 11.75 | 108.36 | 329.81 |
CV(%) | 4.9% | 5.4% | 6.4% | 5% | 7.2% | 7.4% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.033 | 0.055 | 0.111 | 0.207 | 0.399 | 0.735 | 1.251 | 2.029 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)