指标别名
C reactive protein; CRP; PTX1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
C-reactive protein, pentraxin-related
文献引用格式
Human CRP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1316)
背景资料
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1316-CAP | 96-well plate precoated with anti- human CRP antibody | 1 |
| EK1316-ST | Lyophilized recombinant human CRP standard | 20ng/tube×2 |
| EK1316-DA | Biotinylated anti- human CRP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 593 | 1488 | 8142 | 565 | 1551 | 8792 |
| Standard deviation | 36.17 | 62.49 | 643.21 | 38.42 | 75.99 | 861.61 |
| CV(%) | 6.1 | 4.2 | 7.9 | 6.8 | 4.9 | 9.8 |
典型数据示例
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.031 | 0.089 | 0.156 | 0.339 | 0.644 | 1.077 | 1.676 | 2.044 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)