Catalog number | Description | Quantity |
---|---|---|
EK1316-CAP | 96-well plate precoated with anti- human CRP antibody | 1 |
EK1316-ST | Lyophilized recombinant human CRP standard | 20ng/tube×2 |
EK1316-DA | Biotinylated anti- human CRP antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 593 | 1488 | 8142 | 565 | 1551 | 8792 |
Standard deviation | 36.17 | 62.49 | 643.21 | 38.42 | 75.99 | 861.61 |
CV(%) | 6.1 | 4.2 | 7.9 | 6.8 | 4.9 | 9.8 |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.005 | 0.046 | 0.100 | 0.174 | 0.448 | 0.830 | 1.374 | 2.159 |
Human CRP ELISA Kit standard curve
Human CRP ELISA Kit standard curve