Catalog number | Description | Quantity |
---|---|---|
EK0979-CAP | 96-well plate precoated with anti- human IL-31 antibody | 1 |
EK0979-ST | Lyophilized recombinant human IL-31 standard | 10ng/tube×2 |
EK0979-DA | Biotinylated anti- human IL-31 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 134 | 804 | 1907 | 140 | 740 | 1811 |
Standard deviation | 8.04 | 44.22 | 122.04 | 10.5 | 52.54 | 130.39 |
CV(%) | 6 | 5.5 | 6.4 | 7.5 | 7.1 | 7.2 |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.052 | 0.084 | 0.116 | 0.178 | 0.330 | 0.671 | 1.348 | 2.462 |
Human IL-31 ELISA Kit standard curve
Human IL-31 ELISA Kit standard curve