Catalog number | Description | Quantity |
---|---|---|
EK1100-CAP | 96-well plate precoated with anti- mouse IL-31 antibody | 1 |
EK1100-ST | Lyophilized recombinant mouse IL-31 standard | 10ng/tube×2 |
EK1100-DA | Biotinylated anti- mouse IL-31 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 209 | 790 | 1270 | 212 | 719 | 1315 |
Standard deviation | 9.82 | 60.83 | 55.88 | 11.66 | 58.95 | 68.38 |
CV(%) | 4.7% | 7.7% | 4.4% | 5.5% | 8.2% | 5.2% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.033 | 0.081 | 0.130 | 0.220 | 0.408 | 0.793 | 1.392 | 2.219 |
Mouse IL-31 ELISA Kit standard curve
Mouse IL-31 ELISA Kit standard curve