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Human CCL14 ELISA Kit

说明书

EK1123

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human CCL14 ELISA Kit
规格/价格
96T/2480
指标别名
C C motif chemokine 14; CC 1; CC 3; CCL14; Chemokine CC 1/CC 3; CKb1; HCC 1; HCC 1(1 74); HCC 1/HCC 3; HCC 3; MCIF; NCC 2; NCC2; SCYA14; SCYL2; Small inducible cytokine A14; SY14
免疫原
E.coli, T20-N93
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
CCL14
基因名全称
chemokine (C-C motif) ligand 14
Uniprot ID
Q16627  
文献引用格式
Human CCL14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1123)
背景资料
Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1123-CAP96-well plate precoated with anti- human CCL14 antibody1
EK1123-STLyophilized recombinant human CCL14 standard10ng/tube×2
EK1123-DABiotinylated anti- human CCL14 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3723844540227446
Standard deviation1.9213.825.812.0815.8933.45
CV(%)5.25.85.85.277.5
典型数据示例
Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0070.0690.1610.3590.6701.2881.9552.234
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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