产品名称
Human CCL14 ELISA Kit
指标别名
C C motif chemokine 14; CC 1; CC 3; CCL14; Chemokine CC 1/CC 3; CKb1; HCC 1; HCC 1(1 74); HCC 1/HCC 3; HCC 3; MCIF; NCC 2; NCC2; SCYA14; SCYL2; Small inducible cytokine A14; SY14
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
chemokine (C-C motif) ligand 14
文献引用格式
Human CCL14 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1123)
背景资料
Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1123-CAP | 96-well plate precoated with anti- human CCL14 antibody | 1 |
EK1123-ST | Lyophilized recombinant human CCL14 standard | 10ng/tube×2 |
EK1123-DA | Biotinylated anti- human CCL14 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 37 | 238 | 445 | 40 | 227 | 446 |
Standard deviation | 1.92 | 13.8 | 25.81 | 2.08 | 15.89 | 33.45 |
CV(%) | 5.2 | 5.8 | 5.8 | 5.2 | 7 | 7.5 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.007 | 0.069 | 0.161 | 0.359 | 0.670 | 1.288 | 1.955 | 2.234 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)