产品名称
Human CCL14 Quick ELISA Kit
指标别名
C C motif chemokine 14; CC 1; CC 3; CCL14; Chemokine CC 1/CC 3; CKb1; HCC 1; HCC 1(1 74); HCC 1/HCC 3; HCC 3; MCIF; NCC 2; NCC2; SCYA14; SCYL2; Small inducible cytokine A14; SY14
免疫原
Expression system for standard: E.coli; Immunogen sequence: T20-N93
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
chemokine (C-C motif) ligand 14
文献引用格式
Human CCL14 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1123)
背景资料
Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
试剂盒内容
Catalog number | Description | Quantity |
FEK1123-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK1123-ST | Human CCL14 standard | 10ng/tube×2 |
FEK1123-A | Human CCL14 Antibody A | 3ml |
FEK1123-B | Human CCL14 Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 42 | 232 | 450 | 45 | 232 | 451 |
Standard deviation | 2.14 | 12.99 | 25.20 | 2.97 | 17.16 | 34.72 |
CV(%) | 5.1% | 5.6% | 5.6% | 6.6% | 7.4% | 7.7% |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
O.D. | 0.023 | 0.092 | 0.163 | 0.208 | 0.563 | 1.085 | 1.851 | 2.381 |
特异性
Natural and recombinant human CCL14
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)