产品名称
Human Surfactant protein D/SFTPD ELISA Kit
指标别名
COLEC7; Collectin 7; Lung surfactant protein D; PSP D; PSPD; SFTP4; SFTPD; SP D; surfactant protein D
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
625 pg/ml - 40000 pg/ml
基因名全称
surfactant protein D
文献引用格式
Human Surfactant protein D/SFTPD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1171)
背景资料
Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1171-CAP | 96-well plate precoated with anti- human SP-D antibody | 1 |
EK1171-ST | Lyophilized recombinant human SP-D standard | 40ng/tube×2 |
EK1171-DA | Biotinylated anti- human SP-D antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1547 | 3580 | 25269 | 1591 | 3588 | 23717 |
Standard deviation | 92.82 | 221.96 | 1693.02 | 111.37 | 279.86 | 1683.9 |
CV(%) | 6 | 6.2 | 6.7 | 7 | 7.8 | 7.1 |
典型数据示例
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.061 | 0.073 | 0.108 | 0.213 | 0.399 | 0.766 | 1.370 | 2.099 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)