产品名称
Mouse Surfactant protein D/SFTPD ELISA Kit
指标别名
COLEC7; Collectin 7; Lung surfactant protein D; PSP D; PSPD; SFTP4; SFTPD; SP D; surfactant protein D
免疫原
Expression system for standard: CHO; Immunogen sequence: A20-F374
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Pulmonary surfactant-associated protein D
文献引用格式
Mouse Surfactant protein D/SFTPD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1186)
背景资料
Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1186-CAP | 96-well plate precoated with anti- mouse SP-D antibody | 1 |
EK1186-ST | Lyophilized recombinant mouse SP-D standard | 20ng/tube×2 |
EK1186-DA | Biotinylated anti- mouse SP-D antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 641 | 2014 | 9766 | 618 | 1957 | 9758 |
Standard deviation | 26.92 | 151.05 | 625.02 | 37.69 | 162.43 | 692.81 |
CV(%) | 4.2% | 7.5% | 6.4% | 6.1% | 8.3% | 7.1% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.054 | 0.248 | 0.416 | 0.674 | 0.928 | 1.438 | 1.953 | 2.224 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)