产品名称
Mouse Lactadherin/MFGE8 ELISA Kit
指标别名
BA46; Breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; Lactadherin; MFG E8; MFGE8; MFG-E8; MFGM; Milk fat globule EGF factor 8; OAcGD3S; SED1; SPAG10
免疫原
Expression system for standard: NS0; Immunogen sequence: A23-C463
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Lactadherin/MFGE8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1194)
背景资料
MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1194-CAP | 96-well plate precoated with anti- mouse MFGE8 antibody | 1 |
EK1194-ST | Lyophilized recombinant mouse MFGE8 standard | 10ng/tube×2 |
EK1194-DA | Biotinylated anti- mouse MFGE8 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 111 | 677 | 1516 | 120 | 624 | 1633 |
Standard deviation | 6.66 | 45.35 | 98.54 | 9 | 41.8 | 130.64 |
CV(%) | 6% | 6.7% | 6.5% | 7.5% | 6.7% | 8% |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.030 | 0.079 | 0.129 | 0.211 | 0.379 | 0.701 | 1.258 | 1.968 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)