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Mouse Lactadherin/MFGE8 ELISA Kit

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EK1194

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse Lactadherin/MFGE8 ELISA Kit
规格/价格
96T/2480
指标别名
BA46; Breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; Lactadherin; MFG E8; MFGE8; MFG-E8; MFGM; Milk fat globule EGF factor 8; OAcGD3S; SED1; SPAG10
免疫原
Expression system for standard: NS0; Immunogen sequence: A23-C463
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5pg/ml-4000pg/ml
基因名称
MFGE8
基因名全称
Lactadherin
Uniprot ID
P21956  
文献引用格式
Mouse Lactadherin/MFGE8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1194)
背景资料
MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
试剂盒内容
Catalog numberDescriptionQuantity
EK1194-CAP96-well plate precoated with anti- mouse MFGE8 antibody1
EK1194-STLyophilized recombinant mouse MFGE8 standard10ng/tube×2
EK1194-DABiotinylated anti- mouse MFGE8 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11167715161206241633
Standard deviation6.6645.3598.54941.8130.64
CV(%)6%6.7%6.5%7.5%6.7%8%
典型数据示例
Concentration(pg/ml)062.5125250500100020004000
O.D.0.0300.0790.1290.2110.3790.7011.2581.968
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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    Mouse Lactadherin/MFGE8 ELISA Kit

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