Human Lactadherin/MFGE8 ELISA Kit

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EK1201

96T ￥2480
货期：现货

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Human MFGE8/Lactadherin ELISA Kit standard curve
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产品名称

Human Lactadherin/MFGE8 ELISA Kit

规格/价格

96T/2480

指标别名

BA46; Breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; Lactadherin; MFG E8; MFGE8; MFG-E8; MFGM; Milk fat globule EGF factor 8; OAcGD3S; SED1; SPAG10

免疫原

NSO, L24-C387

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

62.5 pg/ml - 4000 pg/ml

基因名称

MFGE8

基因名全称

milk fat globule-EGF factor 8 protein

Uniprot ID

Q08431

文献引用格式

Human Lactadherin/MFGE8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1201)

背景资料

MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK1201-CAP	96-well plate precoated with anti- human MFGE8 antibody	1
EK1201-ST	Lyophilized recombinant human MFGE8 standard	10ng/tube×2
EK1201-DA	Biotinylated anti- human MFGE8 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	100	660	1196	101	717	1149
Standard deviation	6.3	36.96	90.89	8.28	41.58	102.26
CV(%)	6.3	5.6	7.6	8.2	5.8	8.9

典型数据示例

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.052	0.114	0.151	0.234	0.442	0.824	1.445	2.153

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

应用范围

ELISA

检验物种

human

检测方法

酶联免疫双抗夹心法

样本类型

Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk

Human Lactadherin/MFGE8 ELISA Kit

EK1201

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

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