产品名称
Human BIGH3/TGFBI ELISA Kit
指标别名
Beta ig h3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato epithelin; LCD1; RGD CAP; TGFBI; TGFBI / BIGH3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
transforming growth factor, beta-induced, 68kDa
文献引用格式
Human BIGH3/TGFBI ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1202)
背景资料
Transforming growth factor, beta-induced, 68kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1202-CAP | 96-well plate precoated with anti- human TGFBI antibody | 1 |
EK1202-ST | Lyophilized recombinant human TGFBI standard | 10ng/tube×2 |
EK1202-DA | Biotinylated anti- human TGFBI antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 154 | 388 | 1132 | 160 | 387 | 1210 |
Standard deviation | 6.77 | 28.32 | 57.73 | 7.36 | 35.21 | 70.18 |
CV(%) | 4.4 | 7.3 | 5.1 | 4.6 | 9.1 | 5.8 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.044 | 0.068 | 0.107 | 0.173 | 0.353 | 0.674 | 1.198 | 2.028 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)