产品名称
Rat BIGH3/TGFBI ELISA Kit
指标别名
Beta ig h3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato epithelin; LCD1; RGD CAP; TGFBI; TGFBI / BIGH3
免疫原
Expression system for standard: NS0; Immunogen sequence: G24-G685
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
transforming growth factor, beta-induced, 68kDa
文献引用格式
Rat BIGH3/TGFBI ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1571)
背景资料
Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
试剂盒内容
Catalog number | Description | Quantity |
EK1571-CAP | 96-well plate precoated with anti- rat beta IG-H3antibody | 1 |
EK1571-ST | Lyophilized recombinant rat beta IG-H3 standard | 10ng/tube×2 |
EK1571-DA | Biotinylated anti- rat beta IG-H3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 308 | 1355 | 5623 | 336 | 1296 | 5160 |
Standard deviation | 20.63 | 73.17 | 314.88 | 24.19 | 84.24 | 309.6 |
CV(%) | 6.7% | 5.4% | 5.6% | 7.2% | 6.5% | 6% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.010 | 0.031 | 0.064 | 0.137 | 0.376 | 0.922 | 1.879 | 2.599 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)