产品名称
Mouse Neuropilin-1/NRP1 ELISA Kit
指标别名
neuropilin 1; BDCA4; CD304; NP1; NRP; VEGF165R; Neuropilin-1; NRP1
免疫原
Expression system for standard: NS0; Immunogen sequence: F22-P856
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Neuropilin-1/NRP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1342)
背景资料
NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1342-CAP | 96-well plate precoated with anti- mouse Neuropilin-1 antibody | 1 |
EK1342-ST | Lyophilized recombinant mouse Neuropilin-1 standard | 50ng/tube×2 |
EK1342-DA | Biotinylated anti- mouse Neuropilin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1119 | 5985 | 20843 | 1206 | 6427 | 19936 |
Standard deviation | 85.04 | 442.89 | 1354.79 | 96.48 | 533.44 | 1375.58 |
CV(%) | 7.6% | 7.4% | 6.5% | 8% | 8.3% | 6.9% |
典型数据示例
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.027 | 0.149 | 0.304 | 0.538 | 0.923 | 1.365 | 1.861 | 2.363 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)