Human Neuropilin-1/NRP1 ELISA Kit

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EK1353

96T ￥2480
货期：现货

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Human Neuropilin-1 ELISA Kit standard curve
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产品简介>

产品名称

Human Neuropilin-1/NRP1 ELISA Kit

规格/价格

96T/2480

指标别名

neuropilin 1; BDCA4; CD304; NP1; NRP; VEGF165R; Neuropilin-1; NRP1

免疫原

NSO, F22-K644

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

0.78 ng/ml - 50 ng/ml

基因名称

NRP1

基因名全称

neuropilin 1

Uniprot ID

O14786

文献引用格式

Human Neuropilin-1/NRP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1353)

背景资料

NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK1353-CAP	96-well plate precoated with anti- human Neuropilin-1 antibody	1
EK1353-ST	Lyophilized recombinant human Neuropilin-1 standard	50ng/tube×2
EK1353-DA	Biotinylated anti- human Neuropilin-1 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1578	4783	20934	1501	4697	20813
Standard deviation	69.43	315.67	921.09	90.06	371.06	1207.15
CV(%)	4.4	6.6	4.4	6	7.9	5.8

典型数据示例

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.179	0.230	0.259	0.323	0.474	0.817	1.264	1.825

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Neuropilin-1/NRP1 ELISA Kit

EK1353

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(1) All Species Human(1) 清除

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