产品名称
Human ANGPTL3 ELISA Kit
指标别名
ANG 5; Angiopoietin 5; angiopoietin like 3; Angiopoietin like protein 3; Angiopoietin related protein 3; ANGPT5; ANGPTL3
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
angiopoietin-like 3
文献引用格式
Human ANGPTL3 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1374)
背景资料
ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1374-CAP | 96-well plate precoated with anti- human ANGPTL3 antibody | 1 |
EK1374-ST | Lyophilized recombinant human ANGPTL3 standard | 20ng/tube×2 |
EK1374-DA | Biotinylated anti- human ANGPTL3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 704 | 1869 | 9766 | 766 | 1705 | 9664 |
Standard deviation | 32.38 | 110.27 | 556.66 | 46.72 | 121.05 | 570.17 |
CV(%) | 4.6 | 5.9 | 5.7 | 6.1 | 7.1 | 5.9 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.096 | 0.220 | 0.286 | 0.505 | 0.814 | 1.279 | 1.970 | 2.589 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)