产品名称
Human ANGPTL3 Quick ELISA Kit
指标别名
ANG 5; Angiopoietin 5; angiopoietin like 3; Angiopoietin like protein 3; Angiopoietin related protein 3; ANGPT5; ANGPTL3
免疫原
Expression system for standard: NS0; Immunogen sequence: S17-E460
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
angiopoietin-like 3
文献引用格式
Human ANGPTL3 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1374)
背景资料
ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
试剂盒内容
Catalog number | Description | Quantity |
FEK1374-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK1374-ST | Human ANGPTL3 standard | 10ng/tube×2 |
FEK1374-A | Human ANGPTL3 Antibody A | 3ml |
FEK1374-B | Human ANGPTL3 Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 704 | 1869 | 9766 | 766 | 1705 | 9664 |
Standard deviation | 32.38 | 110.27 | 556.66 | 46.72 | 121.05 | 570.17 |
CV(%) | 4.6% | 5.9% | 5.7% | 6.1% | 7.1% | 5.9% |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.000 | 0.106 | 0.183 | 0.340 | 0.554 | 0.918 | 1.632 | 2.188 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)