产品名称
Human CD5L ELISA Kit
指标别名
AIM; API6; CD5 antigen like; CD5 molecule like; CD5L; CT 2; IgM associated peptide; PRO229; SP ALPHA; Spalpha
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human CD5L ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1413)
背景资料
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1413-CAP | 96-well plate precoated with anti- human CD5L antibody | 1 |
EK1413-ST | Lyophilized recombinant human CD5Lstandard | 10ng/tube×2 |
EK1413-DA | Biotinylated anti- human CD5L antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 245 | 1172 | 5450 | 224 | 1288 | 5192 |
Standard deviation | 10.04 | 79.69 | 414.2 | 13.21 | 91.44 | 415.36 |
CV(%) | 4.1 | 6.8 | 7.6 | 5.9 | 7.1 | 8 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.091 | 0.145 | 0.231 | 0.379 | 0.547 | 1.009 | 1.503 | 2.241 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)