产品名称
Mouse CD5L Quick ELISA Kit
指标别名
AIM; API6; CD5 antigen like; CD5 molecule like; CD5L; CT 2; IgM associated peptide; PRO229; SP ALPHA; Spalpha
免疫原
Expression system for standard: NS0; Immunogen sequence: E22-V352
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse CD5L Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1414)
背景资料
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
试剂盒内容
Catalog number | Description | Quantity |
FEK1414-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK1414-ST | Mouse CD5L standard | 10ng/tube×2 |
FEK1414-A | Mouse CD5L Antibody A | 3ml |
FEK1414-B | Mouse CD5L Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 201 | 1789 | 4940 | 207 | 1935 | 4821 |
Standard deviation | 13.26 | 110.91 | 375.44 | 17.38 | 131.58 | 414.6 |
CV (%) | 6.6% | 6.2% | 7.6% | 8.4% | 6.8% | 8.6% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10,000 |
O.D. | 0.091 | 0.145 | 0.231 | 0.379 | 0.547 | 1.009 | 1.503 | 2.241 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)