Mouse ELA2/ELANE ELISA Kit

96T/2480

Bone marrow serine protease; ELA2; ELANE; Elastase 2; elastase 2; neutrophil; GE; HLE; HNE; Human leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase

Expression system for standard: NS0; Immunogen sequence: S27-R260

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

Elane

Neutrophil elastase

Q3UP87  

Mouse ELA2/ELANE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1445)

Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Catalog number	Description	Quantity
EK1445-CAP	96-well plate precoated with anti- mouse Elastase antibody	1
EK1445-ST	Lyophilized recombinant mouse Elastase standard	10ng/tube×2
EK1445-DA	Biotinylated anti- mouse Elastase antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	97	301	903	95	322	978
Standard deviation	7.66	15.95	69.53	7.69	19.32	76.28
CV(%)	7.9%	5.3%	7.7%	8.1%	6%	7.8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.008	0.087	0.171	0.295	0.574	1.100	1.981	2.761

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

mouse

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h