Human ELA2/ELANE ELISA Kit

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EK2064

96T ￥2800
货期：现货

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Human Elastase/ELANE/ELA2 ELISA Kit Standard Curve
all(2)
all(2)

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产品简介>

产品名称

Human ELA2/ELANE ELISA Kit

规格/价格

96T/2800

指标别名

Bone marrow serine protease; ELA2; ELANE; Elastase 2; elastase 2; neutrophil; GE; HLE; HNE; Human leukocyte elastase; Medullasin; NE; Neutrophil elastase; PMN E; PMN elastase

免疫原

NSO, I30-H267

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

0.78 ng/ml - 50 ng/ml

基因名称

ELANE

基因名全称

elastase, neutrophil expressed

Uniprot ID

P08246

文献引用格式

Human ELA2/ELANE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2064)

背景资料

In humans, neutrophil elastase is encoded by the ELANE gene, which resides on chromosome 19. It is mapped to 19p13.3. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK2064-CAP	96-well plate precoated with anti- human Elastase/ELANE/ELA2 antibody	1
EK2064-ST	Lyophilized recombinant human Elastase/ELANE/ELA2 standard	50ng/tube×2
EK2064-DA	Biotinylated anti- human Elastase/ELANE/ELA2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	466	1663	9923	528	1713	9943
Standard deviation	22.37	86.48	535.84	26.93	94.22	626.41
CV(%)	4.8	5.2	5.4	5.1	5.5	6.3

典型数据示例

Concentration(pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.095	0.103	0.122	0.145	0.243	0.534	1.274	2.298

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human ELA2/ELANE ELISA Kit

EK2064

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

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Human ELA2/ELANE ELISA Kit

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