产品名称
Human Fibulin-3/EFEMP1 ELISA Kit
指标别名
DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
312 pg/ml - 20000 pg/ml
基因名全称
EGF containing fibulin-like extracellular matrix protein 1
文献引用格式
Human Fibulin-3/EFEMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1468)
背景资料
EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1468-CAP | 96-well plate precoated with anti- human Fibulin-3 antibody | 1 |
EK1468-ST | Lyophilized recombinant human Fibulin-3 standard | 20ng/tube×2 |
EK1468-DA | Biotinylated anti- human Fibulin-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 650 | 2080 | 10283 | 629 | 2055 | 10529 |
Standard deviation | 31.2 | 85.28 | 473.01 | 37.11 | 117.13 | 610.68 |
CV(%) | 4.8 | 4.1 | 4.6 | 5.9 | 5.7 | 5.8 |
典型数据示例
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.021 | 0.070 | 0.116 | 0.252 | 0.502 | 0.880 | 1.534 | 2.226 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)