产品名称
Mouse Fibulin-3/EFEMP1 ELISA Kit
指标别名
DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; EGF-containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5
免疫原
Expression system for standard: CHO; Immunogen sequence: Q18-F493
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
epidermal growth factor-containing fibulin-like extracellular matrix protein 1
文献引用格式
Mouse Fibulin-3/EFEMP1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1590)
背景资料
EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1590-CAP | 96-well plate precoated with anti- mouse EFEMP1 antibody | 1 |
EK1590-ST | Lyophilized recombinant mouse EFEMP1 standard | 20ng/tubex2 |
EK1590-DA | Biotinylated anti- mouse EFEMP1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 3087 | 14945 | 46910 | 3111 | 1500 | 4713 |
Standard deviation | 145.09 | 821.98 | 2767.69 | 199.1 | 100.5 | 353.48 |
CV(%) | 4.7% | 5.5% | 5.9% | 6.4% | 6.7% | 7.5% |
典型数据示例
Concentration(ng/ml) | 0 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.090 | 0.171 | 0.254 | 0.383 | 0.658 | 1.082 | 1.586 | 2.236 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)