产品名称
Human BMP9/GDF2 ELISA Kit
指标别名
BMP 9; BMP9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
growth differentiation factor 2
文献引用格式
Human BMP9/GDF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1560)
背景资料
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1560-CAP | 96-well plate precoated with anti- human BMP-9 antibody | 1 |
EK1560-ST | Lyophilized recombinant human BMP-9 standard | 10ng/tube×2 |
EK1560-DA | Biotinylated anti- human BMP-9 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 186 | 405 | 34 | 178 | 376 |
Standard deviation | 2.06 | 9.3 | 19.44 | 2.48 | 11.92 | 24.44 |
CV(%) | 5.9 | 5 | 4.8 | 7.3 | 6.7 | 6.5 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.038 | 0.157 | 0.215 | 0.329 | 0.564 | 0.961 | 1.660 | 2.324 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum Plasma (heparin, EDTA) and Urine