产品名称
Mouse BMP9/GDF2 ELISA Kit
指标别名
BMP 9; BMP9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2
免疫原
Expression system for standard: CHO; Immunogen sequence: S319-R428
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
growth differentiation factor 2
文献引用格式
Mouse BMP9/GDF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1610)
背景资料
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1610-CAP | 96-well plate precoated with anti- mouse BMP-9 antibody | 1 |
| EK1610-ST | Lyophilized recombinant mouse BMP-9 standard | 10ng/tube×2 |
| EK1610-DA | Biotinylated anti- mouse BMP-9 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 39 | 220 | 419 | 41 | 226 | 402 |
| Standard deviation | 1.71 | 11.22 | 31.42 | 2.05 | 14.46 | 30.95 |
| CV(%) | 4.4% | 5.1% | 7.5% | 5% | 6.4% | 7.7% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.008 | 0.133 | 0.221 | 0.388 | 0.688 | 1.140 | 1.757 | 2.229 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)