产品名称
Mouse Haptoglobin/HP ELISA Kit
指标别名
BP; HAPT; Haptoglobin; HP; HP2 ALPHA 2; HPA1S; Zonulin
免疫原
Expression system for standard: NS0; Immunogen sequence: V19-N347
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Haptoglobin/HP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1689)
背景资料
Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia .
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1689-CAP | 96-well plate precoated with anti- mouse Haptoglobin antibody | 1 |
EK1689-ST | Lyophilized recombinant mouse Haptoglobin standard | 100ng/tubex2 |
EK1689-DA | Biotinylated anti- mouse Haptoglobin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2678 | 12849 | 42168 | 2657 | 13883 | 41875 |
Standard deviation | 200.85 | 976.52 | 1981.89 | 207.24 | 1082.87 | 2093.75 |
CV(%) | 7.5% | 7.6% | 4.7% | 7.8% | 7.8% | 5% |
典型数据示例
Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.028 | 0.089 | 0.137 | 0.242 | 0.436 | 0.885 | 1.754 | 2.512 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)