Human Haptoglobin/HP ELISA Kit

96T/2480

BP; HAPT; Haptoglobin; HP; HP2 ALPHA 2; HPA1S; Zonulin

NSO,V19-N406

There is no detectable cross-reactivity with other relevant proteins.

312pg/ml-20000pg/ml

HP

haptoglobin

P00738  

Human Haptoglobin/HP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1702)

Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family(haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans(HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.

20-25分钟

Catalog number	Description	Quantity
EK1702-CAP	96-well plate precoated with anti- human Haptoglobin antibody	1
EK1702-ST	Lyophilized recombinant human Haptoglobin standard	200ng/tubex2
EK1702-DA	Biotinylated anti- human Haptoglobin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	5711	36664	105148	5766	37146	11013
Standard deviation	291.26	1979.86	4521.36	282.53	2488.78	870.03
CV(%)	5.1	5.4	4.3	4.9	6.7	7.9

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.070	0.332	0.526	0.998	1.329	1.720	1.961	2.389

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)

3.5h