产品名称
Human Annexin A1/ANXA1 ELISA Kit
指标别名
Annexin 1; annexin A1; Annexin I; ANX1; ANXA1; Calpactin 2; Calpactin II; Chromobindin 9; Lipocortin I; LPC1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
文献引用格式
Human Annexin A1/ANXA1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1745)
背景资料
ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK1745-CAP | 96-well plate precoated with anti- human Annexin A1 antibody | 1 |
EK1745-ST | Lyophilized recombinant human Annexin A1 standard | 10ng/tube×2 |
EK1745-DA | Biotinylated anti- human Annexin A1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 299 | 1474 | 5209 | 303 | 1499 | 5311 |
Standard deviation | 12.56 | 64.86 | 276.1 | 17.9 | 92.9 | 414.3 |
CV(%) | 4.2 | 4.4 | 5.3 | 5.9 | 6.2 | 7.8 |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.066 | 0.196 | 0.245 | 0.452 | 0.861 | 1.437 | 1.943 | 2.275 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate )