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Human Annexin A1/ANXA1 ELISA Kit

说明书

EK1745

  • 96T ¥2800
  • 货期: 现货

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产品名称
Human Annexin A1/ANXA1 ELISA Kit
规格/价格
96T/2800
指标别名
Annexin 1; annexin A1; Annexin I; ANX1; ANXA1; Calpactin 2; Calpactin II; Chromobindin 9; Lipocortin I; LPC1
免疫原
NSO, A2-N346
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
ANXA1
基因名全称
annexin A1
Uniprot ID
P04083  
文献引用格式
Human Annexin A1/ANXA1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1745)
背景资料
ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
显色时间
15-20分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK1745-CAP96-well plate precoated with anti- human Annexin A1 antibody1
EK1745-STLyophilized recombinant human Annexin A1 standard10ng/tube×2
EK1745-DABiotinylated anti- human Annexin A1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2991474520930314995311
Standard deviation12.5664.86276.117.992.9414.3
CV(%)4.24.45.35.96.27.8
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0660.1960.2450.4520.8611.4371.9432.275
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate )
实验时长
3.5h

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