产品名称
Human Annexin A1/ANXA1 Quick ELISA Kit
指标别名
Annexin 1; annexin A1; Annexin I; ANX1; ANXA1; Calpactin 2; Calpactin II; Chromobindin 9; Lipocortin I; LPC1
免疫原
Expression system for standard: NS0; Immunogen sequence: A2-N346
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Annexin A1/ANXA1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1745)
背景资料
ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
试剂盒内容
Catalog number | Description | Quantity |
FEK1745-CAP | Anti-Pre-coated 96-well Strip Microplate | 1 |
FEK1745-ST | Human AnnexinA1 standard | 10ng/tube×2 |
FEK1745-A | Human AnnexinA1 Antibody A | 3ml |
FEK1745-B | Human AnnexinA1 Antibody B | 3ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 299 | 1474 | 5209 | 303 | 1499 | 5311 |
Standard deviation | 12.56 | 64.86 | 276.1 | 17.9 | 92.9 | 414.3 |
CV(%) | 4.2% | 4.4% | 5.3% | 5.9% | 6.2% | 7.8% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.056 | 0.128 | 0.173 | 0.274 | 0.458 | 0.833 | 1.436 | 2.197 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)