Human Annexin A1/ANXA1 Quick ELISA Kit

96T/3580

Annexin 1; annexin A1; Annexin I; ANX1; ANXA1; Calpactin 2; Calpactin II; Chromobindin 9; Lipocortin I; LPC1

Expression system for standard: NS0; Immunogen sequence: A2-N346

There is no detectable cross-reactivity with other relevant proteins.

<5pg/ml

156pg/ml-10000pg/ml

ANXA1

annexin A1

P04083

Human Annexin A1/ANXA1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1745)

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

15分钟

Catalog number	Description	Quantity
FEK1745-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK1745-ST	Human AnnexinA1 standard	10ng/tube×2
FEK1745-A	Human AnnexinA1 Antibody A	3ml
FEK1745-B	Human AnnexinA1 Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	299	1474	5209	303	1499	5311
Standard deviation	12.56	64.86	276.1	17.9	92.9	414.3
CV(%)	4.2%	4.4%	5.3%	5.9%	6.2%	7.8%

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.056	0.128	0.173	0.274	0.458	0.833	1.436	2.197

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)