Figure 1. Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
all(13)
Figure 2. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and BRAF-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody M00075-3 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
all(13) 
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
all(13) 
Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# M00075-3).
all(13) 
Figure 5. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 9. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 10. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
all(13) 
Figure 12. Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF .
all(13) 
Figure 13. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody, and then analyzed by flow cytometry.
all(13) | Western blot (WB): | 1:1000~2000 |
| Immunohistochemistry (IHC): | 1:200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |
| Flow cytometry (FCM): | 1:100 |
Figure 1. Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
Figure 2. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and BRAF-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody M00075-3 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# M00075-3).
Figure 5. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 9. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 10. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 12. Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF .
Figure 13. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody, and then analyzed by flow cytometry.
Figure 1. Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
Figure 2. Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and BRAF-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody M00075-3 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Figure 3. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Figure 4. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF(Cat# M00075-3).
Figure 5. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 8. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 9. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 10. Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 11. Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRAF mouse monoclonal antibody. (M00075-3)
Figure 12. Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF .
Figure 13. HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody, and then analyzed by flow cytometry.
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